Journal: Bio-protocol

Article Title: Chloroplast Movement Imaging Under Different Light Regimes With a Hyperspectral Camera

doi: 10.21769/BioProtoc.5541

Figure Lengend Snippet: (A, E) A plate covered with black velvet is used as a support for detached leaves. (B, F) The leaf petiole is covered with a wet paper towel. (C, G) Half of the leaf is then covered with aluminum foil, and the other half with polyester foil. (D, H) After irradiation with blue light for 1 h, materials covering the leaf halves are removed, and a reflectance standard is added so that the leaves are ready for imaging.

Article Snippet: White diffuse reflectance standard (Spectralon or BaSO 4 -based reflectance standard, e.g., Edmund Optics, catalog number: #54-302) 3.

Techniques: Irradiation, Imaging

Journal: Bio-protocol

Article Title: Chloroplast Movement Imaging Under Different Light Regimes With a Hyperspectral Camera

doi: 10.21769/BioProtoc.5541

Figure Lengend Snippet: Leaves detached from dark-adapted plants were irradiated with either 1.6 or 120 µmol·m -2 ·s -1 of blue light for 1 h to induce chloroplast accumulation and avoidance responses, respectively. Half of the leaf remained in the dark-adapted state, as it was covered with aluminum foil during irradiation. (A, B) Mean difference in reflectance spectra between irradiated and darkened leaf halves calculated from hyperspectral images of 23 low- and 31 high-light-irradiated leaves. Note that the y-axis range in panels A and B is different. The standard deviation is indicated in light blue, and the standard error in darker blue. (C, D) Reflectance images of N. benthamiana leaves, calculated as an average of the hyperspectral images in the visible (400–700 nm) range. Spectra in A and B redrawn from Hermanowicz, P. and Łabuz, J. 2025 [20].

Article Snippet: White diffuse reflectance standard (Spectralon or BaSO 4 -based reflectance standard, e.g., Edmund Optics, catalog number: #54-302) 3.

Techniques: Irradiation, Standard Deviation